1-Hpyrazolo[3,4-b]pyridines

ABSTRACT

This invention claims compounds having the formulae ##STR1## where R 1  is hydrogen, loweralkyl, arylloweralkyl, aryl or heteroaryl; R 2  is hydrogen or loweralkyl; R 3  is loweralkyl, loweralkoxyloweralkyl, cycloalkyl, arylloweralkyl or heteroarylmethyl; R 4  is hydrogen or loweralkyl; and Z is O, S, NR 5  or CH═N, R 5  being loweralkyl, phenylloweralkyl or benzensulfonyl. These compounds are useful as intermediates for synthesizing 6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridines which in turn are useful as analgesic and hypotensive agents.

This is a division of application Ser. No. 812,590 filed Dec. 23, 1985,now U.S. Pat. No. 4,622,326.

This invention relates to compounds having the formula ##STR2## whereinR₁ is hydrogen, loweralkyl, arylloweralkyl, aryl or heteroaryl; R₂ ishydrogen or loweralkyl; R₃ is loweralkyl, loweralkoxyloweralkyl,cycloalkyl, arylloweralkyl or heteroarylmethyl; and R₄ is hydrogen orloweralkyl; or a pharmaceutically acceptable acid addition salt thereof,which are useful for alleviating pain and lowering blood pressure,methods for synthesizing them, and pharmaceutical compositionscomprising an effective amount of such a compound.

This invention also relates to compounds of formulas (II) and (III)below ##STR3## where R₁ through R₄ are as defined above and Z is O, S,NR₅ or CH═N, R₅ being loweralkyl, phenylloweralkyl or benzenesulfonyl,which are useful as intermediate compounds for synthesizing thecompounds of Formula I and methods for synthesizing them.

Throughout the specification and the appended claims, a given chemicalformula or name shall encompass all stereo and optical isomers thereofwhere such isomers exist, as well as pharmaceutically acceptable acidaddition salts thereof and solvates thereof such as for instancehydrates.

The following definitions shall apply throughout the specification andthe appended claims.

Unless otherwise stated or indicated, the term loweralkyl denotes astraight or branched alkyl group having from 1 to 6 carbon atoms.Examples of said loweralkyl includes methyl, ethyl, n-propyl,iso-propyl, n-butyl, iso-butyl, sec-butyl, t-butyl and straight- andbranched-chain pentyl and hexyl.

Unless otherwise states or indicated, the term loweralkoxy denotes astraight or branched alkoxy group having from 1 to 6 carbon atoms.Examples of said loweralkoxy include methoxy, ethoxy, n-propoxy,iso-propoxy, n-butoxy, iso-butoxy, sec-butoxy, t-butoxy and straight-and branched-chain pentoxy and hexoxy.

Unless otherwise stated or indicated, the term cycloalkyl shall mean acycloalkyl group of 3 to 7 carbon atoms.

Unless otherwise stated or indicated, the term halogen shall meanfluorine, chlorine, bromine or iodine.

Unless otherwise stated or indicated, the term aryl shall mean anunsubstituted phenyl group or a phenyl group substituted with 1, 2 or 3substituents each of which being independently a loweralkyl group,loweralkoxy group, hydroxy group, trifluoromethyl group, chlorine orfluorine, with the proviso that the aryl group shall not have chlorineor fluorine at the ortho position.

Unless otherwise stated or indicated, the term heteroaryl group shallmean a group having the formula ##STR4## where Y is O, S, NR₆ or CH═N,R₆ being hydrogen, loweralkyl or phenylloweralkyl, and it shall includeall the positional isomers. Thus, for instance, the term shall includeboth 2-furyl and 3-furyl.

The compounds of this invention are prepared by utilizing one or more ofthe steps described below.

Throughout the description of the synthetic steps, the notations R₁, R₂,R₃, R₄, R₅, R₆, Y and Z shall have the respective meanings given aboveunless otherwise stated or indicted.

Preferred synthetic steps used for synthesizing the compounds of thisinvention are schematically shown in FIG. 1. It should be understood,however, that FIG. 1 is merely a schematic representation presented forthe purpose of illustration and it should not be construed too narrowly.

For details of the reaction steps used for preparing compound V depictedin FIG. 1, the reader is referred to H. Hoehn, Th. Denzel and W. Jansen,J. Heterocyclic Chem. 9, 235 (1972), except that in this invention a lowtemperature (about -75° C. to -40° C.) Grignard reaction was used forthe last step as shown in FIG. 1, whereas Hoehn et al. used acadmium-Grignard reagent at a higher temperature.

STEP A

Compound V where R₁ is not H or heteroaryl is reacted with acetone oximesalt (CH₃)₂ ═N--O⁻ K⁺ to afford compound IIA as shown in FIG. 1.Typically, this reaction is conducted by first preparing the potassiumsalt of acetone oxime anion by reacting acetone oxime with potassiumtertiary-butoxide for instance in a suitable solvent such as anhydroustetrahydrofuran (THF) at room temperature and thereafter adding to themixture a solution of compound V in a suitable solvent such as THF andstirring the resultant mixture at room temperature. Sodium salt ofacetone oxime anion may also be used.

STEP B

Compound IIa where R₁ is not hydrogen or heteroacryl is converted tocompound Ia in the presence of H₃ O⁺ as shown in FIG. 1. Typically thisacid-catalyzed transoximation reaction is conducted by heating a mixturecomprising compound IIa and a suitable medium such as water and ethanolas well as a protonic acid such as HCl. Reflux is preferred.

As an alternative to STEPS A and B, the following two steps may also beused. ##STR5##

STEP C

Compound V where R₁ is not hydrogen or heteroaryl is hydrolyzed tocompound VI as shown in FIG. 1. Said hydrolysis is conducted in asuitable medium such as a mixture comprising water and dimethylsulfoxideand in the presence of an alkaline catalyst such as NaOH. A typicalreaction temperatue is about 50° to 100° C.

STEP D

Compound VI where R₁ is not hydrogen or heteroaryl is reacted withhydroxylamine to obtain compound Ia as shown in FIG. 1. This cyclizationreaction is typically conducted by relfuxing a mixture comprisingcompound VI, hydroxylamine hydrochloride and a suitable solvent such aspyridine.

STEP E

Compound IV is reduced with LiAlH₄ to obtain compound VII as shown inFIG. 1. Typically said reduction is conducted in a suitable medium suchas anhydrous THF, diethyl ether or the like at a temperature of about0°-30° C.

STEP F

Compound VII is oxidized to compound Va with manganese dioxide as shownin FIG. 1. Typically said oxidation is conducted in a suitable mediumsuch as benzene or toluene at a temperature of about 80°-120° C.

STEP G

Compound Va is reacted with acetone oxime salt (CH₃)₂ C═N--O⁻ K⁺ toobtain compound IIb as shown in FIG. 1. This step is conducted insubstantially the same manner as STEP A above.

STEP H

Compound IIb is reacted with heteroaryl lithium of formula VIII where Zis O, S, NR₅ or CH═N, R₅ being loweralkyl, phenylloweralkyl orbenzenesulfonyl as mentioned above to obtain compound III as shown inFIG. 1. When Z is CH═N, the organolithium compound VIII is prepared fromn-buthyl lithium and a bromo compound of the formula ##STR6## in asuitable solvent such as anhydrous ether at a temperature of betweenabout -70° C. and -50° C. When Z is O, S or NR₆, compound VIII isprepared from n-butyl lithium and a compound of the formula ##STR7## ina suitable solvent such as anhydrous ether at a temperature of betweenabout -20° C. and +40° C. Thereafter the solution of compound VIIIprepared above is added to the solution a solution of compound IIb in asuitable solvent such as anhydrous THF and the reaction mixture isstirred at a temperature between about -10° C. and 10° C.

When Z is benzenesulfonyl substituted nitrogen, 1-benzenesulfonylpyrrolewhich is used for preparing compound VIII can readily be prepared frompyrrolyl anion and benzenesulfonyl chloride by routine procedure.

STEP I

Compound III is oxidized to compound VIa with pyridinium chlorochromateto obtain compound VIa as shown in FIG. 1. This oxidation is typicallyconducted by adding pyridinium chlorochromate to a solution of compoundVIa in a suitable solvent such as dichloromethane and stirring themixture at a temperature of about 10°-50° C.

STEP J

Compound VIa is reacted with hydroxylamine to obtain compound Ib asshown in FIG. 1. This step is conducted in substantially the same manneras STEP D.

STEP K

Although not indicated in FIG. 1, when the group Z is benzenesulfonylsubstituted nitrogen in formula Ib, the benzenesulfonyl group can beconverted to hydrogen by hydrolyzing the compound Ib. Said hydrolysis isconducted typically in the presence of NaOH or KOH and a suitable mediumsuch as lower aliphatic alcohol at a temperature of about 80°-120° C.

As a result of STEP J and STEP K, compound I where R₁ is a heteroarylgroup of the formula ##STR8## where Y is O, S, NR₆ or CH═N, R₆ beinghydrogen, loweralkyl or phenylloweralyl are obtained.

STEP L

Compound IIb is hydrolyzed to obtain compound Ic as shown in FIG. 1.This step is conducted in substantially the same manner as STEP B above.

STEP M

Where the group R₁ in the target compound I is a phenyl groupsubstituted with one or more hydroxy groups, said compound is preparedby first synthesizing the corresponding methoxy substituted compoundaccording to the reaction scheme described above and then converting themethoxy group or groups to hydroxy group or groups by cleavage reaction.Said cleavage reaction is typically conducted by refluxing a mixturecomprising the methoxy compound, boron tribormide and a suitable solventsuch as 1,2-dichloroethane and thereafter pouring the reaction mixtureinto ice water.

The 6H-isoxazolo[5,4-d]pyrazolo[3,4-d]pyridines of formula I of thepresent invention are useful as antihypertensive agents due to theirability to depress blood pressure in mammals. Antihypertensive activityis measured in the spontaneous hypertensive rat by the indirect tailcuff method described in "Methods in Pharmacology", A. Schwartz, Ed.,Vol. I, Appleton-Century Crofts, New York, N.Y., 1971, p. 135. In thisprocedure a group of five animals are treated orally for three days withthe test compound in relation to a control group of the same number. Thedrop in blood pressure is measured on the third day followingadministration. The antihypertensive activities of some of thecompounds, expressed as a decrease in mean arterial blood pressure (inmmHg), are given in Table I.

                  TABLE I                                                         ______________________________________                                        ANTIHYPERTENSIVE ACTIVITY                                                                         Antihypertensive SHR                                      Compound            mmHg at 50 mg/kg, PO                                      ______________________________________                                        3-(4-Chlorophenyl)-6-ethyl-6H--                                                                   62                                                        isoxazolo[5,4-d]pyrazolo[3,4-b]-                                              pyridine                                                                      6-Ethyl-3-(4-methoxyphenyl)-6H--                                                                  31                                                        isoxazolo[5,4-d]pyrazolo[3,4-b]-                                              pyridine                                                                      6-Ethyl-3-(3-tolyl)-6H--isoxazolo-                                                                33                                                        [5,4-d]pyrazolo[3,4-b]pyridine                                                (Prior Art Compound)                                                                              40                                                        alpha-Methyldopa                                                              ______________________________________                                    

Compounds I of the present invention are also useful as analgesic agentsdue to their ability to alleviate pain in mammals. The activity of thecompounds is demonstrated in the 2-phenyl-1,4-benzoquinone-inducedwrithing test in mice, a standard assay for analgesia [Proc. Soc. Exptl.Biol. Med., 95, 729 (1957)]. Table 2 shows a result of the test of theanalgesic activities of some of the compounds of this invention.

                  TABLE 2                                                         ______________________________________                                        ANALGESIC ACTIVITY                                                            (Phenylquinone Writhing)                                                                          Analgesic PQW,                                                                % decrease                                                Compound            at 20 mg/kg, SC                                           ______________________________________                                        6-Ethyl-3-tolyl-6H--isoxazolo[5,4-d]-                                                             48                                                        pyrazolo[3,4-b]pyridine                                                       6-Ethyl-3-(4-fluorophenyl)-6H--                                                                   31                                                        isoxazolo[5,4-d]pyrazolo[3,4-b]-                                              pyridine                                                                      6-Ethyl-3-(3-fluorophenyl)-6H--                                                                   55                                                        isoxazolo[5,4-d]pyrazolo[3,4-b]-                                              pyridine                                                                      6-Ethyl-3-(3-hydroxyphenyl)-6H--                                                                  48                                                        isoxazolo[5,4-d]pyrazolo[3,4-b]-                                              pyridine                                                                      6-Ethyl-3-(2-pyridyl)-6H--isoxazolo-                                                              42                                                        [5,4-d]pyrazolo[3,4-b]pyridine                                                (Prior Art Compound)                                                                              ED.sub.50 = 3.9 mg/kg, s.c.                               Propoxyphene                                                                  ______________________________________                                    

Effective quantities of the compounds of the invention may beadministered to a patient by any of the various methods, for example,orally as in capsules or tablets, parenterally in the form of sterilesolutions or suspensions, and in some cases intravenously in the form ofsterile solutions. The free base final products, while effectivethemselves, may be formulated and administered in the form of theirpharmaceutically acceptable acid addition salts for purposes ofstability, convenience of crystallization, increased solubility and thelike.

Acids useful for preparing the pharmaceutically acceptable acid additionsalts of the invention include inorganic acids such as hydrochloric,hydrobromic, sulfuric, nitric, phosphoric and perchloric acids, as wellas organic acids such as tartaric, citric, acetic, succinic, maleic,fumaric and oxalic acids.

The active compounds of the present invention may be orallyadministered, for example, with an inert diluent or with an ediblecarrier, or they may be enclosed in gelatin capsules, or they may becompressed into tablets. For the purpose of oral therapeuticadministration, the active compounds of the invention may beincorporated with excipients and used in the form of tablets, troches,capsules, elixirs, suspensions, syrups, wafers, chewing gum and thelike. These preparations should contain at least 0.5% of activecompound, but may be varied depending upon the particular form and mayconveniently be between 4% to about 70% of the weight of the unit. Theamount of active compound in such compositions is such that a suitabledosage will be obtained. Preferred compositions and preparationsaccording to the present invention are prepared so that an oral dosageunit form contains between 1.0-300 milligrams of active compound.

The tablets, pills, capsules, troches and the like may also contain thefollowing ingredients: a binder such as micro-crystalline cellulose, gumtragacanth or gelatin; an excipient such as starch or lactose, adisintegrating agent such as alginic acid, Primogel, cornstarch and thelike; a lubricant such as magnesium stearate or Sterotex; a glidant suchas colloidal silicon dioxide; and a sweetening agent such as sucrose orsaccharin may be added or a flavoring agent such as peppermint, methylsalicylate, or orange flavoring. When the dosage unit form is a capsule,it may contain, in addition to materials of the above type, a liquidcarrier such as a fatty oil. Other dosage unit forms may contain othervarious materials which modify the physical form of the dosage unit, forexample, as coatings. Thus tablets or pills may be coated with sugar,shellac, or other enteric coating agents. A syrup may contain, inaddition to the active compounds, sucrose as a sweetening agent andcertain preservatives, dyes, coloring and flavors. Materials used inpreparing these various compositions should be pharmaceutically pure andnon-toxic in the amounts used.

For the purposes of parenteral therapeutic administration, the activecompounds of the invention may be incorporated into a solution ofsuspension. These preparations should contain at least 0.1% of activecompound, but may be varied between 0.5 and about 30% of the weightthereof. The amount of active compound in such compositions is such thata suitable dosage will be obtained. Preferred compositions andpreparations according to the present invention are prepared so that aparenteral dosage unit contains between 0.5 and 100 milligrams of activecompound.

The solutions or suspensions may also include the following components:a sterile diluent such as water for injection, saline solution, fixedoils, polyethylene glycols, glycerine, propylene glycol or othersynthetic solvents; antibacterial agents such as benzyl alcohol ormethyl parabens; antioxidants such as ascorbic acid or sodium bisulfite;chelating agents such as ethylenediaminetetraacetic acid; buffers suchas acetates, citrates or phosphates and agents for the adjustment oftonicity such as sodium chloride or dextrose. The parenteral multipledose vials made of glass or plastic.

Examples of the compounds of this invention include:

6-Ethyl-3-phenyl-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine;

6-Ethyl-3-(3-tolyl)-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine;

6-Ethyl-3-(4-tolyl)-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine;

6-Ethyl-3-(3-fluorophenyl)-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine;

6-Ethyl-3-(4-fluorophenyl)-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine;

3-(3-Chlorophenyl)-6-ethyl-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine;

3-(4-Chlorophenyl)-6-ethyl-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine;

6-Ethyl-3-(3-methoxyphenyl)-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine;

6-Ethyl-3-(4-methoxyphenyl)-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine;

6-Ethyl-3-(3-hydroxyphenyl)-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine;

6-Ethyl-3-(2-pyridyl)-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine;

5-Benzoyl-1-ethyl-4-isopropylidenaminooxy-1H-pyrazolo[3,4-b]pyridine;

5-(4-Chlorobenzoyl)-1-ethyl-4-isopropylidenaminooxy-1H-pyrazolo[3,4-b]pyridine;

(1-Ethyl-4-isopropylidenaminooxy-1H-pyrazolo[3,4-b]pyridin-5-yl)(2-pyridyl)carbinol;and

1-Ethyl-4-isopropylidenaminooxy-1H-pyrazolo[3,4-b]pyridine-5-carboxaldehyde

EXAMPLE 1 6-Ethyl-3-phenyl-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine

5-Benzoyl-1-ethyl-4-isopropylideneaminooxy-1H-pyrazolo [3,4-b]pyridine(2.50 g) was refluxed for 2 hours in a mixture prepared from 100 ml of5% hydrochloric acid and 15 ml of ethanol. The reaction mixture was thenextracted into ether and washed consecutively with 5% sodium hydroxidesolution and water. Evaporation of the organic phase gave 1.65 g ofproduct. An analytical sample recrystallized from ethyl acetate/methanolhad mp 151°-153° C.

    ______________________________________                                        ANALYSIS:                                                                     ______________________________________                                        Calculated for C.sub.15 H.sub.12 N.sub.4 O:                                                  68.17% C  4.58% H  21.20% N                                    Found:         68.23% C  4.65% H  21.25% N                                    ______________________________________                                    

EXAMPLE 2 6-Ethyl-3-(3-tolyl)-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine

1-Ethyl-4-hydroxy-5-(3-methylbenzoyl)-1H-pyrazolo[3,4-b]pyridine (4.25g) was refluxed overnight in 75 ml of pyridine containing 4.5 g ofhydroxylamine hydrochloride. The pyridine was then removed under reducedpressure and the residue triturated with 5% hydrochloric acid. Theproduct was then filtered off and recrystallized from methanol to give2.63 g of analytically pure product, mp 122°-123° C.

    ______________________________________                                        ANALYSIS:                                                                     ______________________________________                                        Calculated for C.sub.16 H.sub.15 N.sub.4 O:                                                  69.05% C  5.07% H  20.13% N                                    Found:         68.78% C  5.19% H  20.24% N                                    ______________________________________                                    

EXAMPLE 3 6-Ethyl-3-(4-tolyl)-5H-isoxazolo[3,4-d]pyrazolo[3,4-b]pyridine

1-Ethyl-4-hydroxy-5-(4-methylbenzoyl)-1H-pyrazolo[3,4-b]pyridine (1.45g) was refluxed overnight in 20 ml of pyridine containing 3.0 g ofhydroxylamine hydrochloride. The pyridine was then evaporated and theresidue triturated with 5% hydrochloric acid and then washed withmethanol. In this manner 1.26 g of product was obtained, mp 173°-175° C.An analytical sample was recrystallized from dichloromethane/hexane andthe melting point was unchanged.

    ______________________________________                                        ANALYSIS:                                                                     ______________________________________                                        Calculated for C.sub.16 H.sub.14 N.sub.4 O.sub.4 :                                            69.05% C 5.07% H   20.13% N                                   Found:          68.88% C 5.14% H   30.23% N                                   ______________________________________                                    

EXAMPLE 46-Ethyl-3-(3-fluorophenyl)-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine

1-Ethyl-5-(3-fluorobenzoyl)-4-hydroxy-1H-pyrazolo[3,4-b]pyridine (2.85g) was refluxed overnight in 50 ml of pyridine containing 3.0 g ofhydroxylamine hydrochloride. The pyridine was evaporated and the residuetriturated with 5% hydrochloric acid. The product thus obtained wasfiltered off and recrystallized from methanol to give 2.21 g ofanalytically pure product, mp 136°-137° C.

    ______________________________________                                        ANALYSIS:                                                                     ______________________________________                                        Calculated for C.sub.15 H.sub.11 FN.sub.4 O:                                                  63.82% C 3.93% H   19.85% N                                   Found:          63.92% C 4.05% H   19.90% N                                   ______________________________________                                    

EXAMPLE 56-Ethyl-3-(4-fluorophenyl)-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine

1-Ethyl-5-(4-fluorobenzoyl)-4-hydroxy-1H-pyrazolo[3,4-b]pyridine (3.35g) was refluxed overnight in 75 ml of pyridine containing 4.0 g ofhydroxylamine hydrochloride. The solvent was then evaporated and theresidue triturated with 5% hydrochloric acid. The product thus obtainedwas washed with methanol and then chromatographed over silica gel (5%methanol/dichloromethane) to remove a trace of fluorescent impurity.After recrystallization from ethyl acetate/hexane, 2.83 g of product wasobtained, mp 175°-176° C.

    ______________________________________                                        ANALYSIS:                                                                     ______________________________________                                        Calculated for C.sub.15 H.sub.11 FN.sub.4 O:                                                  63.82% C 3.93% H   19.85% N                                   Found:          63.80% C 3.93% H   20.10% N                                   ______________________________________                                    

EXAMPLE 63-(3-Chlorophenyl)-6-ethyl-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine

5-(3-Chlorobenzoyl)-1-ethyl-4-hydroxy-1H-pyrazolo[3,4-b]pyridine (4.25g) was refluxed overnight in 100 ml of pyridine containing 4.5 g ofhydroxylamine hydrochloride. The reaction mixture was then evaporatedand the residue triturated with 5% hydrochloric acid. The resultingproduct was then filtered off and washed with methanol.Recrystallization from ethyl acetate gave analytically pure product(1.91 g), mp 178°-179° C.

    ______________________________________                                        ANALYSIS:                                                                     ______________________________________                                        Calculated for C.sub.15 H.sub.11 ClN.sub.4 O:                                                  60.31% C 3.71% H  18.76% N                                   Found:           60.10% C 3.68% H  18.89% N                                   ______________________________________                                    

EXAMPLE 73-(4-Chlorophenyl)-6-ethyl-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine

5-(4-chlorobenzoyl)-1-ethyl-4-isopropylideneaminooxy-1H-pyrazolo-[3,4-b]pyridine(3.54 g) was refluxed for 2 hours in 75 ml of 2:1 5% hydrochloricacid/ethanol. The reaction mixture was distributed between water andether and then the ether layer was washed with 5% sodium hydroxide.Evaporation of the ether gave 1.30 g of product. An analytical samplewas recrystallized from hexane which had mp 195°-197° C.

    ______________________________________                                        ANALYSIS:                                                                     ______________________________________                                        Calculated for C.sub.15 H.sub.11 ClN.sub.4 O:                                                  60.31% C 3.71% H  18.76% N                                   Found:           60.68% C 3.83% H  18.64% N                                   ______________________________________                                    

EXAMPLE 86-Ethyl-3-(3-methoxyphenyl)-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine

1-Ethyl-4-hydroxy-5-(3-methoxybenzoyl)-1H-pyrazolo-[3,4-b]pyridine wasrefluxed overnight in 75 ml of pyridine containing 7.0 g ofhydroxylamine hydrochloride. The pyridine was evaporated and the residuetriturated with 5% hydrochloric acid. The product thus obtained wasrecrystallized from methanol to give 5.94 g, mp 130°-131° C.

    ______________________________________                                        ANALYSIS:                                                                     ______________________________________                                        Calculated for C.sub.16 H.sub.14 N.sub.4 O.sub.2 :                                            65.29% C 4.79% H   19.04% N                                   Found:          65.16% C 4.75% H   19.15% N                                   ______________________________________                                    

EXAMPLE 96-Ethyl-3-(4-methoxyphenyl)-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine

1-Ethyl-4-hydroxy-5-(4-methoxybenzoyl)-1H-pyrazolo[3,4-b]pyridine (3.60g) was refluxed overnight in 30 ml of pyridine containing 4.0 g ofhydroxylamine hydrochloride. At the end of this time the pyridine wasevaporated and the residue triturated with 5% hydrochloric acid. Theproduct obtained in this manner was chromatographed silica gel (5%methanol/dichloromethane) to remove a fluorescent impurity. Combinationof the appropriate fractions and recrystallization fromdichloromethane/hexane gave 2.95 g of pure product, mp 156°-157° C.

    ______________________________________                                        ANALYSIS:                                                                     ______________________________________                                        Calculated for C.sub.16 H.sub.14 N.sub.4 O.sub.2 :                                            65.29% C 4.79% H   19.04% N                                   Found:          64.87% C 4.84% H   19.08% N                                   ______________________________________                                    

EXAMPLE 106-Ethyl-3-(3-hydroxyphenyl)-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine

6-Ethyl-3-(3-methoxyphenyl)-6H-isoxazolo[5,4-d]pyrazolo[3,4-d]pyridine(3.84 g) was dissolved in 75 ml of 1,2-dichloroethane and to this wasadded 7.5 ml of BBr₃ (19.9 g). The reaction mixture was then refluxedfor 3 hours, at which time thin layer chromatography showed completereaction. The reaction mixture was poured into ice and the precipitatedproduct was filtered off and recrystallized from methanol, giving 3.14 gof analytically pure product, mp 187°-188° C.

    ______________________________________                                        ANALYSIS:                                                                     ______________________________________                                        Calculated for C.sub.15 H.sub.12 N.sub.4 O.sub.2 :                                            64.28% C 4.32% H   19.99% N                                   Found:          64.27% C 4.32% H   19.73% N                                   ______________________________________                                    

EXAMPLE 116-Ethyl-3-(2-pyridyl)-6H-isoxazolo[5,4-d]pyrazolo[3,4-b]pyridine

(1-Ethyl-4-isopropylideneaminooxy-1H-pyrazolo[3,4-b]pyridine-4-yl)(2-pyridyl)carbinol (8.3 g) was dissolved in 150 ml of dichloromethaneand to this was added 6.5 g of pyridinium chlorochromate. The reactionmixture was stirred 1 hour and then 300 ml of ether was added, afterwhich the mixture was filtered through a pad of magnesium silicate. Thepad was washed with a total of 2 liters of ether and then with 1 literof 5% methanol/dichloromethane. Concentration of the combined filtrateunder a reduced pressure gave 5.8 g of the1-ethyl-4-hydroxy-5-(2-pyridinyl)-1H-pyrazolo[3,4-b]pyridine, whoseidentity was confirmed by its mass spectrum.

Some of this ketone (3.3 g) was heated overnight at 100° in 50 ml ofpyridine containing 3.0 g of hydroxylamine hydrochloride. At the end ofthis time the pyridine was evaporated and the residue triturated with 5%hydrochloric acid to give a solid amorphous product, amounting to 1.6 g,mp 146°-147°. This product was combined with the products of severalother runs and flushed over a silica gel column with 1:1 ethylacetate/dichloromethane. Recrystallization of the product obtained inthis manner from cyclohexane gave analytically pure material, mp151°-153° C.

    ______________________________________                                        ANALYSIS:                                                                     ______________________________________                                        Calculated for C.sub.14 H.sub.11 N.sub.5 O:                                                  63.39% C  4.18% H  26.40% N                                    Found:         63.59% C  4.31% H  26.77% N                                    ______________________________________                                    

EXAMPLE 125-Benzoyl-1-ethyl-4-isopropylidenaminooxy-1H-pyrazolo[3,4-b]pyridine

In 25 ml of dry tetrahydrofuran (THF hereafter) was dissolved 1.6 g ofacetone oxime, followed by 2.46 of potassium t-butoxide. After stirring0.5 hour 5.0 g of 5-benzoyl-4-chloro-1-ethyl-1H-pyrazolo[3,4-b]pyridinein 50 ml of THF was added to the solution. After an additional 0.5 hourthe reaction was quenched with ammonium chloride solution and theproduct was extracted with ether. Evaporation of the organic phase andrecrystallization of the residue from ethanol gave 4.1 g of product, mp115°-116° C.

    ______________________________________                                        ANALYSIS:                                                                     ______________________________________                                        Calculated for C.sub.18 H.sub.18 N.sub.4 O.sub.2 :                                            67.06% C 5.63% H   17.38% N                                   Found:          66.80% C 5.73% H   17.29% N                                   ______________________________________                                    

EXAMPLE 135-(4-Chlorobenzoyl)-1-ethyl-4-isopropylidenaminooxy-1H-pyrazolo[3,4-b]pyridine

Acetone oxime (1.6 g) was dissolved in 50 ml of THF and potassiumt-butoxide (2.46 g) was added. After stirring 30 minutes 6.2 g of4-chloro-5-(4-chlorobenzoyl)-1-ethyl-1H-pyrazolo[3,4-b]pyridine in 50 mlof THF was added to the solution. After 30 minutes of further stirring,the reaction mixture was distributed between ether and ammonium chloridesolution. Drying and evaporation of the organic phase gave a solid whichwas recrystallized from ethanol to give 4.91 g of product, mp 112°-113°C.

    ______________________________________                                        ANALYSIS:                                                                     ______________________________________                                        Calculated for C.sub.18 H.sub.17 ClN.sub.4 O.sub.2 :                                           60.59% C 4.80% H  15.70% N                                   Found:           60.75% C 5.09% H  15.75% N                                   ______________________________________                                    

EXAMPLE 14(1-Ethyl-4-isopropylidenaminooxy-1H-pyrazolo[3,4-b]pyridin-4-yl)(2-pyridyl)carbinol

Twenty-five ml of 1.44M n-BuLi was dissolved in 100 ml of anhydrousether and chilled to -65°. 2-Bromopyridine (5.70 g) was then addeddropwise and stirring was continued for 30 minutes. This solution wasthen added under nitrogen pressure through a catheter to 8.30 g of1-ethyl-5-formyl-4-isopropylidenaminoxy-1H-pyrazolo[3,4-b]pyridine in100 ml of THF at ice bath temperature. This reaction mixture was stirredfor additional 30 minutes and then distributed between 5% hydrochloricacid and ether. The aqueous phase was washed well with ether and thenmade basic by pouring it over solid sodium bicarbonate. The product wasextracted with dichloromethane and purified by preparative HPLC (5%methanol-dichloromethane, 200 ml/minute) to give 6.20 g of product, mp131°-133° C. Recrystallization from dichloromethane-hexane raised the mpto 134°-135° C.

    ______________________________________                                        ANALYSIS:                                                                     ______________________________________                                        Calculated for C.sub.17 H.sub.19 N.sub.5 O.sub.2 :                                            62.75% C 5.89% H   21.53% N                                   Found:          62.52% C 6.06% H   21.43% N                                   ______________________________________                                    

EXAMPLE 15 4-Chloro-1-ethyl-1H-pyrazolo[3,4-b]pyridine-5-carboxaldehyde

Wet MnO₂ (110 g) was refluxed for 90 minutes in 1 L of toluene with aDean-Stark trap for the separation of water. This suspension was thencooled and the 4-chloro-1-ethyl-1H-pyrazolo[3,4-b]pyridine-5-methanol(17.5 g, 82.7 mmole) was added in 50 ml of toluene. The reaction mixturewas then stirred for 6 hours and filtered with the aid of a filter pad.The toluene was evaporated and the residue added again to 100 g of wetMnO₂ freshly prepared in the above manner. After additional 4 hours thereaction was briefly warmed at 55° and then filtered again through afilter pad. The residue which remained after evaporation of the toluenewas purified by preparative high performance liquid chromatography (10%ethyl acetate/hexane, 250 ml/min) to yield 11.70 g of4-chloro-1-ethyl-1H-pyrazolo[3,4-b]pyridine-5-carboxaldehyde, mp 89°-90°C.

    ______________________________________                                        ANALYSIS:                                                                     ______________________________________                                        Calculated for C.sub.9 H.sub.8 ClN.sub.3 O:                                                   51.56% C 3.85% H   20.05% N                                   Found:          51.18% C 3.79% H   19.84% N                                   ______________________________________                                    

EXAMPLE 161-Ethyl-4-isopropylidenaminooxy-1H-pyrazolo[3,4-b]pyridine-5-carboxaldehyde

Potassium t-butoxide (6.70 g) was added to 4.40 g of acetone oxime in100 ml of dimethylacetamide and the solution stirred for 1 hour. Thismixture was then added as a suspension through a catheter to 12.6 g of4-chloro-1-ethyl-1H-pyrazolo[3,4-b]pyridine-5-carboxaldehyde in 200 mlof THF, chilled with ice/water.

After stirring for additional 30 minutes the reaction was quenched withammonium chloride solution and then distributed between dichloromethaneand water. The organic phase was dried, evaporated and chromatographed(20% ethyl acetate/hexane, 200 ml/min) to give, after combination of theappropriate fractions, 10.15 g of product, mp 85°-87° C.

    ______________________________________                                        ANALYSIS:                                                                     ______________________________________                                        Calculated for C.sub.12 H.sub.14 N.sub.4 O.sub.2 :                                            58.52% C 5.73% H   22.75% N                                   Found:          58.55% C 5.60% H   22.83% N                                   ______________________________________                                    

I claim:
 1. A compound having the formula ##STR9## where R₁ is hydrogen, loweralkyl, arylloweralkyl, aryl or heteroaryl; R₂ is hydrogen or loweralkyl; R₃ is loweralkyl, loweralkoxyloweralkyl, cycloalkyl, arylloweralkyl or heteroarylmethyl; and R₄ is hydrogen or loweralkyl, the term aryl in each occurrence signifying an unsubstituted phenyl group or a phenyl group substituted with 1, 2 or 3 substituents each of which being independently a loweralkyl group, loweralkoxy group, hydroxy group, trifluoromethyl group, chlorine or fluorine, with the proviso that the aryl group shall not have chlorine or fluorine at the ortho position; the term heteroaryl in each occurence signifying a group having the formula ##STR10## where Y is O, S, NR₆ or CH═N, R₆ being hydrogen, loweralkyl or phenylloweralkyl; and the term cycloalkyl signifying a cycloalkyl group of 3 to 7 carbon atoms.
 2. The compound as defined in claim 1, wherein R₁ is aryl or heteroaryl.
 3. The compound as defined in claim 1, where R₂ is hydrogen.
 4. The compound as defined in claim 1, where R₃ is loweralkyl.
 5. The compound as defined in claim 1, where R₄ is hydrogen.
 6. The compound as defined in claim 1, which is 1-ethyl-4-isopropylidenaminooxy-1H-pyrazolo[3,4-b]pyridine-5-carboxaldehyde.
 7. A compond having the formula ##STR11## where Z is O, S, NR₅ or CH═N, R₅ being loweralkyl, phenylloweralkyl or benzenesulfonyl; R₂ is hydrogen or loweralkyl; R₃ is loweralkyl; loweralkoxyloweralkyl, cycloalkyl, arylloweralkyl or heteroarylmethyl; and R₄ is hydrogen or loweralkyl, the term heteroaryl signifying a group having the formula ##STR12## where Y is O, S, NR₆ or CH═N, R₆ being hydrogen, loweralkyl or phenylloweralkyl; and the term cycloalkyl signifying a cycloalkyl group of 3 to 7 carbon atoms.
 8. The compound as defined in claim 2, which is 5-benzoyl-1-ethyl-4-isopropylidenaminooxy-1-H-pyrazolo[3,4-b]pyridine.
 9. The compound as defined in claim 2, which is 5-(4-chlorobenzoyl)-1-ethyl-4-isopropylidenaminooxy-1H-pyrazolo[3,4-b]pyridine.
 10. The compound as defined in claim 7, where Z is CH═N.
 11. The compound as defined in claim 7, where R₂ is hydrogen.
 12. The compound as defined in claim 7, where R₃ is loweralkyl.
 13. The compound as defined in claim 7, where R₄ is hydrogen.
 14. The compound as defined in claim 10, which is (1-ethyl-4-isopropylidenaminooxy-1H-pyrazolo[3,4-b]pyridin-4-yl)(2-pyridyl)carbinol. 